Open AccessSulfoquinovose degraded by pure cultures of bacteria with release of C 3 ‐organosulfonates: complete degradation in two‐member communities
Author(s) -
Denger Karin,
Huhn Thomas,
Hollemeyer Klaus,
Schleheck David,
Cook Alasdair M.
Publication year - 2012
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2011.02477.x
Subject(s) - pseudomonas putida , sulfonate , chemistry , klebsiella oxytoca , sulfur , bacteria , yield (engineering) , sulfate , dhps , citrobacter freundii , pseudomonadaceae , pseudomonas , chromatography , nuclear chemistry , biochemistry , organic chemistry , escherichia coli , enterobacteriaceae , biology , enzyme , sodium , genetics , materials science , plasmodium falciparum , malaria , immunology , metallurgy , gene
Sulfoquinovose ( SQ , 6‐deoxy‐6‐sulfoglucose) was synthesized chemically. An HPLC ‐ ELSD method to separate SQ and other chromophore‐free sulfonates, e.g. 2,3‐dihydroxypropane‐1‐sulfonate ( DHPS ), was developed. A set of 10 genome‐sequenced, sulfonate‐utilizing bacteria did not utilize SQ , but an isolate, P seudomonas putida SQ 1, from an enrichment culture did so. The molar growth yield with SQ was half of that with glucose, and 1 mol 3‐sulfolactate (mol SQ ) −1 was formed during growth. The 3‐sulfolactate was degraded by the addition of P aracoccus pantotrophus NKNCYSA , and the sulfonate sulfur was recovered quantitatively as sulfate. Another isolate, K lebsiella oxytoca T au N 1, could utilize SQ , forming 1 mol DHPS (mol SQ ) −1 ; the molar growth yield with SQ was half of that with glucose. This DHPS could be degraded by Cupriavidus pinatubonensis JMP 134, with quantitative recovery of the sulfonate sulfur as sulfate. We presume that SQ can be degraded by communities in the environment.