Open AccessProbiotic genomic DNA reduces the production of pro‐inflammatory cytokine tumor necrosis factor‐alpha
Author(s) -
Hee Kim Chung,
Geun Kim Han,
Yun Kim Joo,
Ra Kim Na,
Jun Jung Bong,
Hye Jeong Ji,
Kyun Chung Dae
Publication year - 2012
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2011.02470.x
Subject(s) - tumor necrosis factor alpha , toll like receptor 9 , tlr4 , biology , mapk/erk pathway , cytokine , interleukin 1 receptor , kinase , cancer research , microbiology and biotechnology , interleukin , signal transduction , immunology , biochemistry , gene expression , dna methylation , gene
The effect of L actobacillus plantarum genomic DNA on lipopolysaccharide ( LPS )‐induced mitogen‐activated protein kinase ( MAPK ) activation, nuclear factor‐kappa B activation, and the expressions of tumor necrosis factor‐alpha, interleukin‐1 receptor‐associated kinase M , and the pattern recognition receptor were examined. Pretreatment of p‐ gDNA inhibited the phosphorylation of MAPK s and nuclear factor‐kappa B , and also inhibited LPS ‐induced TNF ‐α production in response to subsequent LPS stimulation. L . plantarum genomic DNA ‐mediated inhibition of signaling pathway and tumor necrosis factor‐alpha was accompanied by the suppression of toll‐like receptor ( TLR ) 2, TLR 4, and TLR 9 and the induction of interleukin‐1 receptor‐associated kinase M , a negative regulator of TLR . This study can extend our understanding of the biological function of probiotic genomic DNA as an anti‐inflammatory agent.