Open AccessC orynebacterium glutamicum whcB , a stationary phase‐specific regulatory gene
Author(s) -
Lee JooYoung,
Park JoonSong,
Kim HyungJoon,
Kim Younhee,
Lee HeungShick
Publication year - 2012
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2011.02463.x
Subject(s) - corynebacterium glutamicum , gene , biology , corynebacterium , biochemistry , regulation of gene expression , function (biology) , transfection , phenotype , plasmid , microbiology and biotechnology , bacteria , chemistry , genetics
The function of whcB , one of the four whiB homologues of C orynebacterium glutamicum , was assessed. Cells carrying the P 180 ‐ whcB clone, and thus overexpressing the whcB gene, showed retarded growth, probably due to increased sensitivity to oxidants, whereas cells lacking whcB ( Δ whcB ) did not. However, growth retardation was not observed in cells with additionally whcE deleted. Furthermore, the Δ whcE phenotype, characterized by slow growth and sensitivity to oxidants, was reversed in cells carrying P 180 ‐ whcB . Like the whcE gene, which is also known as a whiB homologue, the whcB gene was preferentially expressed in stationary phase. Determination of the genes under regulation of whcB using two‐dimensional polyacrylamide gel electrophoresis identified several genes involved in electron transfer reactions that were regulated in cells carrying P 180 ‐ whcB . Collectively, these findings indicate that whcB function requires whcE . Furthermore, whcB and whcE are paralogues but perform distinct regulatory roles during growth under oxidative stress.