Open AccessEvaluation of the catalase promoter for expressing the alkaline xylanase gene ( alx ) in A spergillus niger
Author(s) -
Sharma Ruchika,
Katoch Meenu,
Govindappa Nagraj,
Srivastava P. S.,
Sastry Kedarnath N.,
Qazi Ghulam Nabi
Publication year - 2012
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2011.02454.x
Subject(s) - aspergillus niger , catalase , promoter , heterologous expression , xylanase , gene , gene expression , microbiology and biotechnology , heterologous , recombinant dna , biochemistry , biology , chemistry , expression vector , enzyme
A spergillus niger represents a promising host for the expression of recombinant proteins, but only a few expression systems are available for this organism. In this study, the inducible catalase promoter ( P cat R ) from A . niger was characterized. For this, constructs were developed and checked for the expression of the alkaline xylanase gene transcriptionally fused under the cat R promoter. Two versions of the catalase ( catR ) promoter sequence from A . niger ( P cat300, P cat924 ) were isolated and tested for their ability to drive expression of the alkaline xylanase ( alx ) gene. P cat924 showed better efficiency (more than 10‐fold increase in AlX activity compared to P cat300 ) under the optimized culture conditions. Induction of the catR promoter with 0.20% H 2 O 2 and 1.5% CaCO 3 in the culture medium, further increased expression of AlX 2.61‐ and 2.20‐fold, respectively, clarifying its inducible nature. Specific induction or repression of the catR promoter provides the possibility for utilization of this promoter in heterologous protein production.