Open AccessS tenotrophomonas interspecies differentiation and identification by gyrB sequence analysis
Author(s) -
SvenssonStadler Liselott A.,
Mihaylova Sashka A.,
Moore Edward R.B.
Publication year - 2012
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2011.02452.x
Subject(s) - stenotrophomonas maltophilia , stenotrophomonas , biology , dna gyrase , sequence analysis , 16s ribosomal rna , genetics , gene , microbiology and biotechnology , genomic dna , genotyping , dna sequencing , ribosomal rna , genotype , bacteria , escherichia coli , pseudomonas aeruginosa
S tenotrophomonas species are found commonly in environmental and clinical samples; S tenotrophomonas maltophilia is an important opportunistic pathogen of humans. Traditional phenotyping protocols, as well as genotyping by 16 S rRNA gene sequence analysis, do not reliably distinguish the species of S tenotrophomonas . Sequence analyses of two targeted PCR ‐amplified regions of the gyrB gene, which encodes the β‐subunit of DNA gyrase, enabled resolution and identification of these species. Most type strains of the different species of S tenotrophomonas exhibited more than 7% dissimilarity in the gyrB gene sequences. Among these, strains identified as the same species exhibited sequence dissimilarities up to 4.6% and 5.9% for the two regions, respectively. Strains identified as S . maltophilia , with 16S rRNA gene sequence similarities > 99.0%, were grouped within a ‘ S . maltophilia complex’; these organisms exhibited gyrB similarities as low as 93%. Many of these strains possessed genomic DNA similarities with the type strain of S . maltophilia CCUG 5866 T below 70%. These data, including gyrB sequence comparisons, indicate that strains identified as S . maltophilia may comprise distinct, new species.