Open AccessPreliminary molecular identification of cylindrospermopsin‐producing C yanobacteria in two P olish lakes ( C entral E urope)
Author(s) -
MankiewiczBoczek Joanna,
Kokociński Mikołaj,
Gagała Ilona,
Pawełczyk Jakub,
Jurczak Tomasz,
Dziadek Jarosław
Publication year - 2012
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2011.02451.x
Subject(s) - cylindrospermopsin , cylindrospermopsis raciborskii , aphanizomenon , cyanobacteria , biology , cyanotoxin , botany , microcystin , bacteria , anabaena , genetics
The presence of toxigenic cyanobacteria capable of biosynthesis of cylindrospermopsin ( CYN ) was measured in 24 water samples collected from the lakes B ytyńskie ( BY ) and B nińskie ( BN ) in the W estern P oland. The study also covered analysis of toxigenicity and production of CYN by the culture of C ylindrospermopsis raciborskii isolated from BY . The cyrJ gene associated with CYN production was identified in 22 water samples collected in the summer seasons of 2006 and 2007. The presence of CYN was confirmed in 16 samples. The homology searches revealed that amplified sequences of four water samples, which were selected from among all the samples, displayed a strong 99% homology to cyrJ gene of A phanizomenon sp. 10 E 6. The culture of C . raciborskii did not contain the cyrJ gene nor the CYN . The specificity of C . raciborskii was confirmed by application of a fragment of the rpoC1 . These first genetic analyses have shown that A phanizomenon seems to be the main cyanobacterial genus responsible for the production of CYN in the P olish lakes. The lack of toxigenicity of the isolated C . raciborskii suggests that it is possible that this invasive species does not demonstrate toxigenic activity in P olish water bodies.