Open AccessSerum resistance in H aemophilus parasuis SC 096 strain requires outer membrane protein P 2 expression
Author(s) -
Zhang Bin,
Feng Saixiang,
Xu Chenggang,
Zhou Suming,
He Yanbing,
Zhang Lingyun,
Zhang Jianmin,
Guo Lili,
Liao Ming
Publication year - 2012
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2011.02433.x
Subject(s) - bacterial outer membrane , haemophilus , microbiology and biotechnology , virulence , strain (injury) , mutant , biology , serotype , virulence factor , phenotype , bacteria , membrane protein , escherichia coli , transformation (genetics) , chemistry , gene , membrane , biochemistry , genetics , anatomy
H aemophilus parasuis outer membrane protein P 2 ( OmpP2 ), the most abundant protein in the outer membrane, has been identified as an antigenic protein and a potential virulence factor. To study the precise function of OmpP2 , an ompP2 ‐deficient mutant (Δ ompP2 ) of a H . parasuis serovar 4 clinical strain SC 096 was constructed by a modified natural transformation system. Compared with the wild‐type SC 096 strain, the Δ ompP2 mutant showed a pronounced growth defect and exhibited significantly greater sensitivity to the bactericidal action of porcine and rabbit sera, whereas the complemented strain could restore the growth and serum resistance phenotypes. The results indicated that H . parasuis OmpP2 from SC 096 strain is an important surface protein involved in serum resistance.