Open AccessGenetic analysis of the pnp – deaD genetic region reveals membrane lipoprotein NlpI as an independent participant in cold acclimatization of S almonella enterica serovar T yphimurium
Author(s) -
Rouf Syed Fazle,
Anwar Naeem,
Clements Mark O.,
Rhen Mikael
Publication year - 2011
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2011.02416.x
Subject(s) - salmonella enterica , salmonella , operon , biology , acclimatization , gene , microbiology and biotechnology , bacterial outer membrane , genetics , bacteria , escherichia coli
The cold acclimatization response in many bacterial species is a tightly regulated process, which ensures the correct folding of macromolecules. In enterobacteria, this response is in part dependent on polynucleotide phosphorylase, which is encoded by the gene pnp . Based on transcriptional analysis of the pnp locus of S almonella enterica serovar T yphimurium, we show that pnp and the adjacent membrane lipoprotein nlpI gene form an operon with both genes contributing independently to the cold acclimatization response at 15 °C. Our findings thereby define a new role for NlpI in bacterial cold acclimatization.