Open AccessIsolation of the fenoxaprop‐ethyl ( FE )‐degrading bacterium R hodococcus sp. T 1, and cloning of FE hydrolase gene feh
Author(s) -
Hou Ying,
Tao Jian,
Shen Wenjing,
Liu Juan,
Li Jingquan,
Li Yongfeng,
Cao Hui,
Cui Zhongli
Publication year - 2011
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2011.02376.x
Subject(s) - strain (injury) , hydrolase , chemistry , cosmid , bacteria , enrichment culture , metabolite , propargyl , gene , biochemistry , microbiology and biotechnology , stereochemistry , enzyme , biology , catalysis , genetics , anatomy
An enrichment culture which completely degraded fenoxaprop‐ethyl ( FE ) was acquired by using FE as sole carbon source. An efficient FE ‐degrading strain T 1 was isolated from the enrichment culture and identified as R hodococcus sp. Strain T 1 could degrade 94% of 100 mg L −1 FE within 24 h and the metabolite fenoxaprop acid ( FA ) was identified by HPLC / MS analysis. This strain converted FE by cleavage of the ester bond, but could not further degrade FA . Strain T 1 could also efficiently degrade haloxyfop‐ R ‐methyl, quizalofop ‐p‐ ethyl, cyhalofop‐butyl and clodinafop‐propargyl. FE hydrolase capable of hydrolysing FE to FA was found in the cell‐free extract of strain T 1 by zymogram analysis. A novel gene feh encoding FE hydrolase was cloned by shotgun library construction and successfully expressed in E scherichia coli .