Open AccessThe rhamnolipid stress response of B acillus subtilis
Author(s) -
Wecke Tina,
Bauer Tobias,
Harth Henning,
Mäder Ulrike,
Mascher Thorsten
Publication year - 2011
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2011.02367.x
Subject(s) - bacillus subtilis , rhamnolipid , cell envelope , biology , bacteria , surfactin , gene , microbiology and biotechnology , antimicrobial , transcriptome , response regulator , biofilm , function (biology) , pseudomonas aeruginosa , chemistry , escherichia coli , genetics , gene expression , mutant
Rhamnolipids are biosurfactants produced by the soil bacterium P seudomonas aeruginosa . In addition to their high industrial potential as surface‐active molecules, rhamnolipids also have antimicrobial properties. In densely populated habitats, such as the soil, production of antimicrobial compounds is important to inhibit growth of competitors. For the latter, it is crucial for survival to sense and respond to the presence of those antibiotics. To gain a first insight into the biological competition involving biosurfactants, we investigated the cellular response of the model organism B acillus subtilis upon exposure to rhamnolipids by genome‐wide transcriptional profiling. Most of the differentially expressed genes can be assigned to two different regulatory networks: the cell envelope stress response mediated by the two‐component system LiaRS and the extracytoplasmic function σ factor σ M and the CssRS ‐dependent secretion stress response. Subsequent phenotypic analysis demonstrated a protective function of LiaRS and σ M against cell lysis caused by rhamnolipids. Taken together, we present the first evidence that a single antimicrobial compound can simultaneously induce genes from two independent stress stimulons.