Open AccessL actobacillus alvi sp. nov., isolated from the intestinal tract of chicken
Author(s) -
Kim HeeJin,
Eom SeokJin,
Park SungJoon,
Cha ChangJun,
Kim GeunBae
Publication year - 2011
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2011.02361.x
Subject(s) - lactobacillus , microbiology and biotechnology , chemistry , medicine , biology , food science , fermentation
Strain R54 T was isolated from the gizzard of hens. The isolate was G ram‐positive, facultative anaerobic, gas‐forming, catalase‐negative, nonmotile, nonspore‐forming and short‐rod‐shaped. The optimal temperature for growth was 40 °C and the DNA G+C content was 42.7 mol%. The 16 S rRNA gene sequences similarity showed that strain R54 T was most closely related to L actobacillus ingluviei LMG 20380 T (97.5%), followed by Lactobacillus coleohominis CIP 106820 T (96.1%), Lactobacillus secaliphilus DSM 17896 T (95.6%) and Lactobacillus gastricus LMG 22113 T (95.4%). The DNA–DNA relatedness between strain R54 T and L . ingluviei LMG 20380 T , was 43.3%. The predominant cellular fatty acids of strain R54 T were C 18:1 ω9c (64.9%) and C 16:0 (20.0%), and the major polar lipid group was phospholipids. On the basis of polyphasic taxonomy approach, strain R54 T represents a novel species of the genus L actobacillus , for which the name L actobacillus alvi sp. nov. is proposed (type strain R54 T = KCCM 90099 T = JCM 17644 T ).