BopC is a type III secreted effector protein of B urkholderia pseudomallei

B urkholderia pseudomallei , the causative agent of melioidosis, exploits the B sa type III secretion system ( T3SS ) to deliver effector proteins into host cells. These effectors manipulate host cell functions; thus, contributing to the ability of the bacteria to evade the immune response and cause disease. Only two B sa‐secreted effectors have been conclusively identified to date. Here, we report the identification of the third B . pseudomallei type III secreted effector protein, designated BopC . BopC is encoded by the bpss1516 gene abutting bpss1517 , which encodes its putative chaperone. The genes are located in the close proximity to the bsa T3SS gene cluster of B . pseudomallei K 96243 (Fig. 1). BopC was secreted into culture supernatant by the wild‐type B . pseudomallei strain, but its secretion was abolished in the bsaZ T3SS mutant. Using pull down and co‐purification assays, we confirmed that BopC interacts with its putative chaperone, BPSS 1517, in vitro . Furthermore, the first 20 N ‐terminal amino acids of BopC were found to be sufficient to mediate the T3SS ‐dependent translocation of a reporter protein from a heterologous enteropathogenic E scherichia coli host into mammalian cells. Finally, bopC mutant was found to be less invasive than the wild‐type strain in the epithelial cells.