Open AccessEnzymatic detachment of spore germlings in Magnaporthe oryzae
Author(s) -
Inoue Kanako,
Onoe Takatoshi,
Park Pyoyun,
Ikeda Kenichi
Publication year - 2011
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2011.02353.x
Subject(s) - appressorium , gelatinase , matrix metalloproteinase , collagenase , microbiology and biotechnology , extracellular matrix , gelatinase a , biology , spore , extracellular , adhesion , enzyme , chemistry , biochemistry , organic chemistry
Magnaporthe oryzae germlings tightly attach to the host surface by producing extracellular matrix (ECM) from germ tubes and appressoria, which are important for the early infection process. To understand the adhesion mechanisms of ECM during differentiation of infection structure, we evaluated the effects of various enzymes on M. oryzae germlings and the disease symptoms of the host plant, wheat. Treatment with β‐mannosidase, collagenase N‐2, collagenase S‐1, or gelatinase B at 1‐h postinoculation (hpi) resulted in germling detachment, although producing normal appressoria. Treatment with matrix metalloproteinases (MMPs) at 6 hpi also caused germling detachment. Furthermore, we confirmed by the inoculation tests and scanning electron microscopy that the germlings on the wheat plant were removed and did not manifest pathogenicity on treatment with MMPs. The most effective MMPs were crude collagenase, collagenase S‐1, and gelatinase B, suggesting that the application of MMPs is promising for crop protection from fungal diseases by its detachment action.