Open AccessPurification and characterization of a soluble cytochrome c capable of delivering electrons to chlorate reductase in Ideonella dechloratans
Author(s) -
Bäcklund Anna S.,
Nilsson Thomas
Publication year - 2011
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2011.02321.x
Subject(s) - chlorate , periplasmic space , cytochrome , cytochrome c , reductase , biochemistry , chemistry , electron transport chain , enzyme , inorganic chemistry , mitochondrion , escherichia coli , gene
The electron donor for periplasmic chlorate reductase of Ideonella dechloratans has been suggested to be a soluble cytochrome c . We describe here the purification of the 9‐kDa periplasmic cytochrome c , denoted cytochrome c ‐Id1, and demonstrate its ability to serve as an electron donor for purified chlorate reductase. The reaction rate was found to be linearly dependent on the cytochrome c concentration in the range of 0.6–4 μM. A route for electron transport involving a soluble cytochrome c is similar to that found for other periplasmic oxidoreductases of the dimethyl sulfoxide reductase family, but different from that suggested for the (per)chlorate reductase of Dechloromonas species.