Open AccessA holin remnant protein encoded by STY1365 is involved in envelope stability of Salmonella enterica serovar Typhi
Author(s) -
Rodas Paula I.,
Trombert A. Nicole,
Mora Guido C.
Publication year - 2011
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2011.02310.x
Subject(s) - salmonella enterica , biology , escherichia coli , salmonella typhi , microbiology and biotechnology , phase variation , salmonella , cell envelope , bacteriophage , gene , genetics , bacteria , phenotype
We characterized STY1365, a small ORF of Salmonella enterica serovar Typhi. This 174‐bp ORF encodes a putative product of 57 amino acid residues with a premature stop codon. Nevertheless, bioinformatic analyses revealed that the predicted product of STY1365 has similarity to putative holin genes of Escherichia coli and bacteriophage ΦP27. STY1365 showed a high‐level expression at the early log phase and a small corresponding protein product was detected mainly in the inner membrane fraction. Cloning of STY1365 in pSU19 mid‐copy‐vector produced retardation in S . Typhi growth, increased cell permeability to crystal violet and altered the inner membrane protein profile. Similar results were obtained when STY1365 was induced with isopropyl‐β‐ d ‐thio‐galactoside in pCC1 ™ single‐copy vector. Our results support the fact that S . Typhi STY1365 encodes a holin remnant protein that is involved in the stability of the bacterial envelope.