Open AccessA new subspecies of Anoxybacillus flavithermus ssp. yunnanensis ssp. nov. with very high ethanol tolerance
Author(s) -
Dai Jun,
Liu Yang,
Lei Yin,
Gao Yi,
Han Fang,
Xiao Yazhong,
Peng Hui
Publication year - 2011
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2011.02294.x
Subject(s) - 16s ribosomal rna , biology , subspecies , bacteria , microbiology and biotechnology , strain (injury) , thermophile , xylose , phylogenetic tree , dna–dna hybridization , arabinose , botany , gene , genetics , fermentation , food science , zoology , anatomy
In a search for thermophilic ethanol‐tolerant bacteria, water‐sediment samples collected at springs in Yunnan province of China were screened by ethanol enrichment. A novel thermophilic bacterium, strain E13 T , was isolated. It exhibits a unique and remarkable ability to preferably grow in the presence of ethanol and is able to tolerate 13% (v/v) ethanol at 60 °C. The isolate is a facultative aerobic, Gram‐positive, motile, spore‐forming rod that is capable of utilizing a range of carbon sources, such as xylose, arabinose and cellobiose. Phylogenetic analysis based on 16S rRNA gene similarity showed the strain to be affiliated with the species Anoxybacillus flavithermus (99.2% sequence similarity). DNA–DNA hybridization comparisons demonstrated a 64.8% DNA–DNA relatedness between strain E13 T and A. flavithermus DSM 2641 T . On the basis of phenotypic characteristics, phylogenetic data and DNA–DNA hybridization data, it was concluded that the isolate merited classification as a novel subspecies of A. flavithermus , for which the name Anoxybacillus flavithermus ssp. yunnanensis ssp. nov. is proposed. The type strain of this subspecies is E13 T (=CCTCC AB2010187 T =KCTC 13759 T ).