Open AccessGenes for hydrogen peroxide detoxification and adaptation contribute to protection against heat shock in Xanthomonas campestris pv. campestris
Author(s) -
Buranajitpakorn Sarinya,
Piwkam Ag,
Charoenlap Nisanart,
Vattanaviboon Paiboon,
Mongkolsuk Skorn
Publication year - 2011
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2011.02211.x
Subject(s) - xanthomonas campestris , detoxification (alternative medicine) , hydrogen peroxide , adaptation (eye) , microbiology and biotechnology , xanthomonas campestris pv. campestris , biology , shock (circulatory) , chemistry , botany , gene , biochemistry , medicine , alternative medicine , pathology , neuroscience
Xanthomonas campestris pv. campestris , a soil‐borne plant‐pathogenic bacterium, is exposed to multiple stresses in the environment and during interaction with a host plant. The roles of hydrogen peroxide (H 2 O 2 )‐protective genes ( katA , katG , and ahpC ) and a peroxide sensor/transcription regulator ( oxyR ) in the viability of X. campestris pv. campestris at an elevated temperature were evaluated. The single katA and katG mutants showed moderate decreased survival after the heat treatment, while the double katA‐katG and oxyR mutants were the most vulnerable to the heat treatment compared with a wild‐type strain. However, ahpC provided no protective function against the heat treatment. Flow cytometric analysis revealed an increased accumulation of peroxide in cells treated with heat. Altogether, the data revealed a crucial role of genes in the H 2 O 2 detoxification system for protection against lethal heat shock in X. campestris pv. campestris .