Open AccessHtpS, a novel immunogenic cell surface‐exposed protein of Streptococcus suis , confers protection in mice
Author(s) -
Shao Zhuqing,
Pan Xiuzhen,
Li Xianfu,
Liu Wenjing,
Han Mingyue,
Wang Changjun,
Wang Jing,
Zheng Feng,
Cao Min,
Tang Jiaqi
Publication year - 2011
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2010.02162.x
Subject(s) - streptococcus suis , biology , microbiology and biotechnology , antigen , western blot , streptococcaceae , serotype , antibody , recombinant dna , flow cytometry , gene , virology , virulence , immunology , genetics , antibiotics
Streptococcal histidine triad protein was identified recently as a cell surface‐associated protein family. Five members of this family (PhtA, PhtB, PhtD, PhtE and HtpA), derived from Streptococcus pneumoniae and Streptococcus pyogenes , have been shown as antigens that confer protection to the host on infection. In this report, a gene sequence highly homologous to htp A and pht D (designated htp S, the histidine triad protein of Streptococcus suis ) was identified from S. suis 2 Chinese strain 05ZYH33. Our data revealed that htp S is extremely conserved in S. suis 2 and widely distributed in 83% (29/35) of 35 S. suis serotypes. It was also demonstrated by Western blot and flow cytometry that HtpS is a cell surface‐associated protein that was expressed during S. suis 2 infection. An antibody against HtpS could increase the deposition of human complement 3 on S. suis 2 and also enhance the clearance of S. suis 2 in whole blood. In addition, mice could be immunized against S. suis 2 infection and were well protected after immunization with recombinant HtpS.