Open AccessIdentification of the J and K genes in the bacteriophage Mu genome sequence
Author(s) -
Smith Mikela L.,
Avanigadda Lakshmi N.,
Liddell Patty W.,
Kenwright Kathleen M.,
Howe Martha M.
Publication year - 2010
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2010.02128.x
Subject(s) - orfs , prophage , genetics , transposable element , biology , genome , gene , lysogenic cycle , bacteriophage , bacteriophage mu , lysogen , open reading frame , peptide sequence , escherichia coli
Bacteriophage Mu was the first transposable phage to be discovered and still serves as the model for a large family of related transposable phages and prophages. The Mu genome sequence is known (NC‐000929.1 GI:9633494), but not all of the genes have been assigned to the ORFs in the genome sequence. For this paper, we have sequenced an approximately 3‐kb DNA region containing four predicted ORFs, Mup35–Mup38, from lysogens containing amber mutant prophages defective in either the J or the K gene. Amber mutations in prophages with J gene mutations mapped to the Mup36 ORF, and those in the K gene were found in Mup37, identifying the ORFs corresponding to these genes.