Open AccessCopper ions potentiate organic hydroperoxide and hydrogen peroxide toxicity through different mechanisms in Xanthomonas campestris pv. campestris
Author(s) -
Patikarnmonthon Nisa,
Nawapan Sirikan,
Buranajitpakorn Sarinya,
Charoenlap Nisanart,
Mongkolsuk Skorn,
Vattanaviboon Paiboon
Publication year - 2010
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2010.02124.x
Subject(s) - chemistry , hydrogen peroxide , xanthomonas campestris , lipid peroxidation , antioxidant , hydroxyl radical , bacteria , biochemistry , biology , genetics , gene
Copper (Cu)‐based biocides are important chemical controls for both fungal and bacterial diseases in crop fields. Here, we showed that Cu ions at a concentration of 100 μM enhanced t‐ butyl hydroperoxide ( t BOOH) and hydrogen peroxide (H 2 O 2 ) killing of Xanthomonas campestris pv. campestris through different mechanisms. The addition of an antilipid peroxidation agent (α‐tocopherol) and hydroxyl radical scavengers (glycerol and dimethyl sulphoxide) partially protected the bacteria from the Cu‐enhanced t BOOH and H 2 O 2 killing, respectively. Inactivation of the alkyl hydroperoxide reductase gene rendered the mutant vulnerable to lethal doses of copper sulphate, which could be alleviated by the addition of an H 2 O 2 scavenger (pyruvate) and α‐tocopherol. Taken together, the data suggest that Cu ions influence the killing effect of t BOOH through the stimulation of lipid peroxidation, while hydroxyl radical production is the underlying mechanism responsible for the Cu‐ion‐enhanced H 2 O 2 killing effects.