Open AccessSoluble and particulate methane monooxygenase gene clusters of the type I methanotroph Methylovulum miyakonense HT12
Author(s) -
Iguchi Hiroyuki,
Yurimoto Hiroya,
Sakai Yasuyoshi
Publication year - 2010
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2010.02101.x
Subject(s) - methane monooxygenase , methanotroph , particulates , methane , monooxygenase , chemistry , environmental chemistry , anaerobic oxidation of methane , biology , ecology , biochemistry , cytochrome p450 , enzyme
The gene clusters encoding soluble methane monooxygenase (sMMO) and particulate methane monooxygenase (pMMO) were cloned and sequenced from a new type I methanotroph, Methylovulum miyakonense HT12. The sMMO gene cluster consisted of the structural genes mmoXYBZDC , the regulatory genes mmoG and mmoR and another ORF orf1 . Transcriptional analysis revealed that these sMMO genes were transcribed as a single unit from a σ 54 ‐dependent promoter located upstream of mmoX . In the pMMO gene cluster, the pmoCAB operon was under the control of a σ 70 ‐dependent promoter. The organization of each MMO operon was largely conserved with that of the previously described methanotrophs. However, unlike other methanotrophs, M. miyakonense HT12 harbored only a single copy of the pmoCAB gene. These data provide new insights into the structure of MMO genes.