Open AccessGeneration of ramoplanin‐resistant Staphylococcus aureus
Author(s) -
Schmidt John W.,
Greenough Adrienne,
Burns Michelle,
Luteran Andrea E.,
McCafferty Dewey G.
Publication year - 2010
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2010.02051.x
Subject(s) - staphylococcus aureus , autolysis (biology) , microbiology and biotechnology , vancomycin , lipid ii , nisin , antibiotics , antibiotic resistance , antimicrobial , drug resistance , peptidoglycan , biology , chemistry , bacteria , biochemistry , enzyme , genetics
Ramoplanin is a lipoglycodepsipeptide antimicrobial active against clinically important Gram‐positive bacteria including methicillin‐resistant Staphylococcus aureus . To proactively examine ramoplanin resistance, we subjected S. aureus NCTC 8325‐4 to serial passage in the presence of increasing concentrations of ramoplanin, generating the markedly resistant strain RRSA16. Susceptibility testing of RRSA16 revealed the unanticipated acquisition of cross‐resistance to vancomycin and nisin. RRSA16 displayed phenotypes, including a thickened cell wall and reduced susceptibility to Triton X‐100‐induced autolysis, which are associated with vancomycin intermediate‐resistant S. aureus strains. Passage of RRSA16 for 18 days in a drug‐free medium yielded strain R16‐18d with restored antibiotic susceptibility. The RRSA16 isolate may be used to identify the genetic and biochemical basis for ramoplanin resistance and to further our understanding of the evolution of antibiotic cross‐resistance mechanisms in S. aureus .