Open AccessProduction of minicellulosomes from Clostridium cellulovorans for the fermentation of cellulosic ethanol using engineered recombinant Saccharomyces cerevisiae
Author(s) -
Hyeon Jeongeun,
Yu KyungOk,
Suh Dong Jin,
Suh YoungWoong,
Lee Sung Eun,
Lee Jinwon,
Han Sung Ok
Publication year - 2010
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2010.02035.x
Subject(s) - clostridium thermocellum , cellulase , cellulosome , cellulose , carboxymethyl cellulose , fermentation , biochemistry , chemistry , cellulosic ethanol , heterologous expression , recombinant dna , organic chemistry , sodium , gene
Saccharomyces cerevisiae was engineered for assembly of minicellulosomes by heterologous expression of a recombinant scaffolding protein from Clostridium cellulovorans and a chimeric endoglucanase E from Clostridium thermocellum . The chimeric endoglucanase E fused with the dockerin domain of endoglucanase B from C. cellulovorans was assembled with the recombinant scaffolding protein. The resulting strain was able to ferment amorphous cellulose [carboxymethyl‐cellulose (CMC)] into ethanol with the aid of β‐glucosidase 1 produced from Saccharomycopsis fibuligera . The minicellulosomes assembled in vivo retained the synergistic effect for cellulose hydrolysis. The minicellulosomes containing the cellulose‐binding domain were purified by crystalline cellulose affinity in a single step. In the fermentation test at 10 g L −1 initial CMC, approximately 3.45 g L −1 ethanol was produced after 16 h. The yield (in grams of ethanol produced per substrate) was 0.34 g g −1 from CMC. This result indicates that a one‐step processing of cellulosic biomass in a consolidated bioprocessing configuration is technically feasible by recombinant yeast cells expressing functional minicellulosomes.