Open AccessPrevalence and diversity of insertion sequences in the genome of Bacillus thuringiensis YBT‐1520 and comparison with other Bacillus cereus group members
Author(s) -
Qiu Ning,
He Jin,
Wang Yan,
Cheng Gang,
Li Mingshun,
Sun Ming,
Yu Ziniu
Publication year - 2010
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2010.02033.x
Subject(s) - bacillus thuringiensis , bacillus cereus , cereus , biology , genome , genetics , bacillaceae , insertion sequence , gene , microbiology and biotechnology , bacteria , transposable element , bacillus subtilis
Members of the Bacillus cereus group are closely related bacteria that exhibit highly divergent pathogenic properties. Sequencing of Bacillus thuringiensis ssp. kurstaki strain YBT‐1520 revealed an increased number of insertion sequences (ISs) compared with those of the published B. cereus group genomes. Although some of these ISs have been observed and summarized in B. thuringiensis previously, a genomic characterization of their content is required to reveal their distribution and evolution. The result shows that the larger number of transposase coding genes on YBT‐1520 chromosome is mainly caused by the amplification of IS 231 C, IS 232 A and IS Bth 166. Some functional genes have been disrupted through the insertion of ISs, preferentially IS 231 C. By comparing the Southern hybridization profiles of different B. thuringiensis strains, the existence of IS Bth 166 was mainly found in serovar kurstaki and the recent expansion of IS 231 C between different kurstaki isolates was suggested. In addition to revealing the ISs profile in YBT‐1520 as well as the comparison in the B. cereus group, this study will contribute to further comparative analyses of multiple B. thuringiensis strains aimed at understanding the IS‐mediated genomic rearrangements among them.