Open AccessMucilaginibacter dorajii sp. nov., isolated from the rhizosphere of Platycodon grandiflorum
Author(s) -
Kim ByungChun,
Lee Kang Hyun,
Kim Mi Na,
Lee Jongtae,
Shin KeeSun
Publication year - 2010
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2010.02026.x
Subject(s) - rhizosphere , botany , biology , bacteria , paleontology
A Gram‐negative, nonmotile and rod‐shaped bacterial strain was isolated from the rhizosphere of Platycodon grandiflorum in a study of bacterial diversity, and its taxonomic position was investigated by a genotypic and phenotypic analysis. This isolate, designated as DR‐f4, grew at 4–30 °C (optimally at 20–25 °C) and in the presence of 0–1% (w/v) NaCl. It contained MK‐7 as the predominant menaquinone. The isolate had activities of catalase, oxidase and β‐galactosidase and hydrolyzed aesculin, casein, carboxymethyl‐cellulose, starch and l ‐tyrosine. The major cellular fatty acids were summed feature 3 (C 16:1 ω7c and/or iso‐C 15:0 2OH) and iso‐C 15:0 . The DNA G + C content was 42.6 mol%. This isolate belonged to the genus Mucilaginibacter based on phylogenetic analysis using 16S rRNA gene sequences. The nearest phylogenetic neighbors of strain DR‐f4 T were Mucilaginibacter lappiensis ANJL12 T and Mucilaginibacter rigui WPCB133 T , with 16S rRNA gene sequence similarity levels of 96.9% and 96.4%, respectively. The genotypic and phenotypic evidence suggests that strain DR‐f4 T should be classified as a novel species, for which the name Mucilaginibacter dorajii sp. nov. is proposed. The type strain for the novel species is DR‐f4 T (=KACC 14556 T =JCM 16601 T ).