Open AccessAestuariibacter aggregatus sp. nov., a moderately halophilic bacterium isolated from seawater of the Yellow Sea
Author(s) -
Wang Yan,
Wang Hong,
Liu Jiwen,
Lai Qiliang,
Shao Zongze,
Austin Brian,
Zhang XiaoHua
Publication year - 2010
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2010.02011.x
Subject(s) - 16s ribosomal rna , biology , halophile , bacteria , phosphatidylglycerol , microbiology and biotechnology , seawater , strain (injury) , agar , oxidase test , botany , ribosomal rna , phospholipid , biochemistry , gene , ecology , anatomy , phosphatidylcholine , enzyme , genetics , membrane
A Gram‐negative, non‐spore‐forming, catalase‐ and oxidase‐positive, strictly aerobic, short rod‐shaped bacterium with a single, polar flagellum, designated strain WH169 T , was isolated from seawater of the Yellow Sea in China. Buds and prosthecae were formed when the organism was grown at 20 °C for 12 days on marine 2216E agar. The organism grew optimally at 37 °C, in pH 7.0–8.0, and in the presence of 4.0–6.0% w/v NaCl. Growth did not occur in a medium without Na + or sea salts. Strain WH169 T contained ubiquinone‐8 as the predominant respiratory lipoquinone and C 16:1 ω7c and/or C 16:1 ω6c (35.9%), C 16:0 (25.3%) and C 18:1 ω7c (9.7%) as the major fatty acids. The polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid. The DNA G+C content of strain WH169 T was 49.4 mol%. 16S rRNA gene sequence analysis showed that strain WH169 T showed 95.1% sequence similarity to both type strains of the only two species in the genus Aestuariibacter . On the basis of the polyphasic taxonomic evidence presented in this study, it was concluded that strain WH169 T should be classified as a novel species of Aestuariibacter , for which the name Aestuariibacter aggregatus sp. nov. is proposed, with the type strain WH169 T (=CGMCC 1.8995 T =LMG 25283 T ).