Open AccessDifferentially expressed genes under simulated microgravity in fruiting bodies of the fungus Pleurotus ostreatus
Author(s) -
Miyazaki Yasumasa,
Sunagawa Masahide,
Higashibata Akira,
Ishioka Noriaki,
Babasaki Katsuhiko,
Yamazaki Takashi
Publication year - 2010
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2010.01966.x
Subject(s) - gravitropism , clinostat , biology , pleurotus ostreatus , gene , fungus , suppression subtractive hybridization , botany , transcriptome , microbiology and biotechnology , gene expression , mushroom , genetics , cdna library , arabidopsis , mutant
In response to a change in the direction of gravity, morphogenetic changes of fruiting bodies of fungi are usually observed as gravitropism. Although gravitropism in higher fungi has been studied for over 100 years, there is no convincing evidence regarding the graviperception mechanism in mushrooms. To understand gravitropism in mushrooms, we isolated differentially expressed genes in Pleurotus ostreatus (oyster mushroom) fruiting bodies developed under three‐dimensional clinostat‐simulated microgravity. Subtractive hybridization, cDNA representational difference analysis was used for gene analysis and resulted in the isolation of 36 individual genes (17 upregulated and 19 downregulated) under clinorotation. The phenotype of fruiting bodies developed under simulated microgravity vividly depicted the gravitropism in mushrooms. Our results suggest that the differentially expressed genes responding to gravitational change are involved in several potential cellular mechanisms during fruiting body formation of P. ostreatus .