Open AccessVibrio hippocampi sp. nov., a new species isolated from wild seahorses ( Hippocampus guttulatus )
Author(s) -
Balcázar José Luis,
Pintado José,
Planas Miquel
Publication year - 2010
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2010.01955.x
Subject(s) - biology , vibrio , 16s ribosomal rna , microbiology and biotechnology , phylogenetic tree , strain (injury) , bacteria , gene , genetics , anatomy
A Gram‐negative, facultatively anaerobic, motile and slightly curved rod‐shaped bacterium (BFLP‐4 T ) was isolated from the faeces of wild seahorses ( Hippocampus guttulatus ) captured in northwest Spain (Toralla, Galicia). Strain BFLP‐4 T grew at 10–35 °C and pH 5–9 (optimally at 20 °C and pH 7.2) and at salt concentrations in the range 0–7% w/v NaCl. The G+C content of the DNA was 49.3 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain BFLP‐4 T was a member of the genus Vibrio , being most closely related to Vibrio ichthyoenteri (97.1%), Vibrio mediterranei (96.7%), Vibrio scophthalmi (96.7%) and Vibrio sinaloensis (96.6%). A phylogenetic analysis based on recA gene sequences also supported the affiliation of strain BFLP‐4 T to the genus Vibrio . Strain BFLP‐4 T could be readily differentiated from other closely related species by several phenotypic properties and fatty acid profiles. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain BFLP‐4 T represents a novel species within the genus Vibrio , for which the name Vibrio hippocampi sp. nov. is proposed. The type strain is BFLP‐4 T (=DSM 22717 T =LMG 25354 T ).