Open AccessApplication of real‐time PCR in Ghana, a Buruli ulcer‐endemic country, confirms the presence of Mycobacterium ulcerans in the environment
Author(s) -
Vandelannoote Koen,
Durnez Lies,
Amissah Diana,
Gryseels Sophie,
Dodoo Alfred,
Yeboah Shirley,
Addo Phyllis,
Eddyani Miriam,
Leirs Herwig,
Ablordey Anthony,
Portaels Françoise
Publication year - 2010
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2010.01902.x
Subject(s) - mycobacterium ulcerans , buruli ulcer , biology , mycobacterium , polymerase chain reaction , microbiology and biotechnology , virology , veterinary medicine , medicine , bacteria , disease , pathology , biochemistry , gene , genetics
This study reports the first successful application of real‐time PCR for the detection of Mycobacterium ulcerans , the causative agent of Buruli ulcer (BU), in Ghana, a BU‐endemic country. Environmental samples and organs of small mammals were analyzed. The real‐time PCR assays confirmed the presence of M. ulcerans in a water sample collected in a BU‐endemic village in the Ashanti Region.