Open AccessA reason for overlap peaks in direct sequencing of rRNA gene ITS in Pleurotus nebrodensis
Author(s) -
Huang Chenyang,
Xu Jingyu,
Gao Wei,
Chen Qiang,
Wang Hexiang,
Zhang Jinxia
Publication year - 2010
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2009.01891.x
Subject(s) - internal transcribed spacer , pleurotus , biology , gene , protoplast , genetics , ribosomal rna , dna sequencing , botany , mushroom
To examine why we failed in direct sequencing of rRNA gene internal transcribed spacer (ITS) in Pleurotus nebrodensis , we obtained monokaryons of P. nebrodensis (00489 and 00491) using a protoplast monokaryonization technique. PCR products of ITS amplifications were sequenced. There was a base pair insertion/deletion difference between the two nuclei of P. nebrodensis that led to failure in direct sequencing.