Open AccessIncreased synthesis of signaling molecules coincides with reversible inhibition of nucleolytic activity during postirradiation recovery of Deinococcus radiodurans
Author(s) -
Kamble Vidya A.,
Rajpurohit Yogendra S.,
Srivastava Ashish K.,
Misra Hari S.
Publication year - 2010
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2009.01855.x
Subject(s) - deinococcus radiodurans , adenylyl cyclase , dna repair , biology , dna damage , phosphodiesterase , microbiology and biotechnology , signal transduction , phosphorylation , second messenger system , cyclic adenosine monophosphate , biochemistry , deinococcus , kinase , dna , enzyme , receptor
Deinococcus radiodurans tolerates extensive DNA damage and exhibits differential expression of various genes associated with the growth of the organism and DNA repair. In cells treated with γ radiation, the levels of cyclic AMP (cAMP) and ATP increased rapidly by differentially regulating adenylyl cyclase (AC) and 2′3′ cAMP phosphodiesterase. The levels of cAMP, ATP, AC and protein kinases were high when phosphodiesterase activity was low. These cells exhibited in vivo inhibition of nucleolytic function by reversible protein phosphorylation and contained the comparatively higher levels of total phosphoproteins. We suggest that Deinococcus , a prokaryote, uses DNA damage‐induced signaling mechanism as evidenced by γ radiation‐induced synthesis of secondary messengers and signaling enzymes.