Open AccessSalicylidene acylhydrazide‐mediated inhibition of type III secretion system‐1 in Salmonella enterica serovar Typhimurium is associated with iron restriction and can be reversed by free iron
Author(s) -
Layton Abigail N.,
Hudson Debra L.,
Thompson Arthur,
Hinton Jay C.D.,
Stevens Joanne M.,
Galyov Edouard E.,
Stevens Mark P.
Publication year - 2010
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2009.01847.x
Subject(s) - salmonella enterica , salmonella , secretion , microbiology and biotechnology , biology , regulator , type three secretion system , serotype , pathogen , bacteria , enterobacteriaceae , transcription factor , mutant , gene , escherichia coli , genetics , biochemistry
Salmonella enterica serovar Typhimurium is an animal and zoonotic pathogen of worldwide importance. Intestinal colonization, induction of enteritis and systemic translocation by this bacterium requires type III protein secretion. Strategies that target this process have the potential to control infection, pathology and transmission. We defined the global transcriptional response of S . Typhimurium to INP0403, a member of a family of salicylidene acylhydrazides that inhibit type III secretion (T3S). INP0403 treatment was associated with reduced transcription of genes involved in T3S, but also increased transcription of genes associated with iron acquisition. We show that INP0403 restricts iron availability to Salmonella , and that inhibition of T3S system‐1 by INP0403 is, at least in part, reversible by exogenous iron and independent of the iron response regulator Fur.