Open AccessFast DNA‐based identification of the black truffle Tuber melanosporum with direct PCR and species‐specific primers
Author(s) -
Bonito Gregory
Publication year - 2009
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2009.01812.x
Subject(s) - truffle , biology , polymerase chain reaction , mushroom , botany , edible mushroom , ribosomal dna , gene , genetics , phylogenetic tree
A quick and sensitive DNA‐based assay for identifying the black truffle species Tuber melanosporum is presented. Both direct PCR and species‐specific primers are used to amplify a phylogenetically informative region of T. melanosporum rDNA. This method was successfully used to screen fresh and frozen T. melanosporum fruitbodies, and could be modified for the molecular detection of other truffle and mushroom species.