Open AccessAnalysis of 16S–23S rRNA gene internal transcribed spacer of Vibrio anguillarum and Vibrio ordalii strains isolated from fish
Author(s) -
Fernández Jorge,
AvendañoHerrera Ruben
Publication year - 2009
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2009.01755.x
Subject(s) - vibrio anguillarum , biology , internal transcribed spacer , spacer dna , 23s ribosomal rna , transfer rna , ribosomal rna , genetics , gene , ribosomal intergenic spacer analysis , vibrio , microbiology and biotechnology , rna , bacteria , ribosome
The 16S‐23S rRNA intergenic spacer (ITS) of Vibrio anguillarum and Vibrio ordalii were PCR amplified and cloned with TA vector pCR2.1. PCR amplification obtained five products ranging from 917 to 437 bp. Three clones were obtained and analysed from all fragments with the exception of 437 bp. These products were designated ITS‐1, ITS‐2, ITS‐3 and ITS‐4. ITS‐1 contained genes for tRNA Glu(TTC) , tRNA Lys(TTT) , tRNA Ala(TGC) and tRNA Val(TAC) , while ITS‐2 was almost the same as the ITS‐1 sequence, but without tRNA Val(TAC) . ITS‐3 contained tRNA Ala(TGC) and tRNA Ile (GAT) and ITS‐4, tRNA Ala (GGC) or tRNA Glu(TTC) . The number of copies of the ribosomal operon ( rrn ) in V. ordalii chromosome ranged from at least six to seven and V. anguillarum had at least seven rrn . The sequences ITS‐1, ITS‐2 and ITS‐3 showed a high similarity among the V. anguillarum and V. ordalii sequences (97.2% to 100%). Little variation was found for ITS‐4, which does not seem to be sufficient to distinguish these two closely related species. Based on the findings, we confirm a close genetic relationship among V. anguillarum and V. ordalii and that they may be descended from a common ancestor in the Vibrionaceae linage.