Open AccessProkaryotic diversity in continuous cropping and rotational cropping soybean soil
Author(s) -
Tang Hui,
Xiao Cuihong,
Ma Jinzhu,
Yu Miao,
Li Yumei,
Wang Genlin,
Zhang Liping
Publication year - 2009
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2009.01730.x
Subject(s) - phylotype , biology , 16s ribosomal rna , rhizosphere , diversity index , bacterial phyla , species evenness , terminal restriction fragment length polymorphism , phylum , restriction fragment length polymorphism , species richness , pyrosequencing , community structure , soil microbiology , agronomy , botany , actinobacteria , soil water , ecology , bacteria , genetics , polymerase chain reaction , gene
In spite of the techniques based on the amplification of 16S rRNA genes (16S rDNA) to compare bacterial communities that are now widely in use in microbial ecology, little is known about the composition of the soybean continuous cropping (CC) and rotational cropping (RC) soil microbial community. To address this, we compared the levels of bacterial community diversity in RC and 5‐year CC rhizosphere soil samples. We selected 407 clones in RC and 490 clones in CC for restriction fragment length polymorphism analysis. A total of 123 phylotypes were identified among the 16S rDNA clones, while 78 unique and 21 common phylotypes were identified among the CC soil isolates. Analysis of sequences from a subset of the phylotypes showed that at least 11 bacterial divisions were represented in the clone libraries. The phylotype richness, frequency distribution (evenness), and composition of the two clone libraries were investigated using a variety of diversity indices. Although the analysis of diversity indices and LIBSHUFF comparisons revealed that the compared libraries were not significantly different ( P =0.05) between the RC vs. CC soils, some differences could be observed in terms of specific phyla and groups. We concluded that the group variance was not determined immediately by the cropping system's induction, but was a long‐term and slow process.