Open AccessDeletion of host histone acetyltransferases and deacetylases strongly affects Agrobacterium ‐mediated transformation of Saccharomyces cerevisiae
Author(s) -
Soltani Jalal,
Van Heusden Gerard Paul H.,
Hooykaas Paul J.J.
Publication year - 2009
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2009.01723.x
Subject(s) - saccharomyces cerevisiae , biology , acetyltransferase , acetylation , histone acetyltransferase , transformation (genetics) , agrobacterium , histone acetyltransferases , plasmid , agrobacterium tumefaciens , histone deacetylase , gene , genetics , histone , trichostatin a , histone h2a
Agrobacterium tumefaciens is a plant pathogen that genetically transforms plant cells by transferring a part of its Ti‐plasmid, the T‐strand, to the host cell. Under laboratory conditions, it can also transform cells from many different nonplant organisms, including the yeast Saccharomyces cerevisiae . Collections of S. cerevisiae strains have been developed with systematic deletion of all coding sequences. Here, we used these collections to identify genes involved in the Agrobacterium ‐mediated transformation (AMT) of S. cerevisiae . We found that deletion of genes ( GCN5 , NGG1 , YAF9 and EAF7 ) encoding subunits of the SAGA, SLIK, ADA and NuA4 histone acetyltransferase complexes highly increased the efficiency of AMT, while deletion of genes ( HDA2 , HDA3 and HST4 ) encoding subunits of histone deacetylase complexes decreased AMT. These effects are specific for AMT as the efficiency of chemical (lithium acetate) transformation was not or only slightly affected by these deletions. Our data are consistent with a positive role of host histone deacetylation in AMT.