Open AccessFolate overproduction in Lactobacillus plantarum WCFS1 causes methotrexate resistance
Author(s) -
Wegkamp Arno,
De Vos Willem M.,
Smid Eddy J.
Publication year - 2009
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2009.01690.x
Subject(s) - overproduction , lactobacillus plantarum , methotrexate , microbiology and biotechnology , strain (injury) , lactobacillus , biology , chemistry , bacteria , biochemistry , enzyme , lactic acid , immunology , genetics , anatomy
Folate overproduction can serve as a mode of resistance against the folate antagonist methotrexate in Lactobacillus plantarum WCFS1. When compared with a wild‐type control strain, an engineered high folate‐producing strain was found to be insensitive to methotrexate. The growth rate and the viable count of the folate‐overproducing L. plantarum strain were not significantly affected by the presence of methotrexate in the growth medium.