Open AccessTryptophan auxotrophs were obtained by random transposon insertions in the Methanococcus maripaludis tryptophan operon
Author(s) -
Porat Iris,
Whitman William B.
Publication year - 2009
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2009.01689.x
Subject(s) - methanococcus , operon , transposable element , genetics , trp operon , biology , auxotrophy , tryptophan , transformation (genetics) , chemistry , gene , genome , archaea , amino acid , mutant
Methanococcus maripaludis is an anaerobic, methane‐producing archaeon that utilizes H 2 or formate for the reduction of CO 2 to methane. Tryptophan auxotrophs were constructed by in vitro insertions of the Tn5 transposon into the tryptophan operon, followed by transformation into M. maripaludis . This method could serve for rapid insertions into large cloned DNA regions.