Open AccessSterol content analysis suggests altered eburicol 14α‐demethylase (CYP51) activity in isolates of Mycosphaerella graminicola adapted to azole fungicides
Author(s) -
Bean Tim P.,
Cools Hans J.,
Lucas John A.,
Hawkins Nathaniel D.,
Ward Jane L.,
Shaw Michael W.,
Fraaije Bart A.
Publication year - 2009
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2009.01645.x
Subject(s) - fungicide , mycosphaerella graminicola , azole , sterol , biology , demethylase , graminicola , microbiology and biotechnology , botany , antifungal , biochemistry , pathogen , cholesterol , epigenetics , gene
The recent decline in the effectiveness of some azole fungicides in controlling the wheat pathogen Mycosphaerella graminicola has been associated with mutations in the CYP51 gene encoding the azole target, the eburicol 14α‐demethylase (CYP51), an essential enzyme of the ergosterol biosynthesis pathway. In this study, analysis of the sterol content of M. graminicola isolates carrying different variants of the CYP51 gene has revealed quantitative differences in sterol intermediates, particularly the CYP51 substrate eburicol. Together with CYP51 gene expression studies, these data suggest that mutations in the CYP51 gene impact on the activity of the CYP51 protein.