Open AccessBiodegradation of the sulfonylurea herbicide chlorimuron‐ethyl by the strain Pseudomonas sp. LW3
Author(s) -
Ma JiPing,
Wang Zhe,
Lu Peng,
Wang Huijie,
Waseem Ali Shinawar,
Li ShunPeng,
Huang Xing
Publication year - 2009
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2009.01638.x
Subject(s) - biodegradation , strain (injury) , bioaugmentation , sulfonylurea , pseudomonas , bioremediation , inoculation , chemistry , microbiology and biotechnology , bacteria , soil contamination , microbial biodegradation , degradation (telecommunications) , biology , horticulture , contamination , microorganism , organic chemistry , ecology , telecommunications , genetics , anatomy , insulin , computer science
The chlorimuron‐ethyl‐degrading bacterium LW3 was isolated from contaminated soil and identified by 16S rRNA gene sequencing as Pseudomonas sp. When chlorimuron‐ethyl was provided as the sole nitrogen source, the degradation efficiency in liquid medium was about 81.0% after 7 days of inoculation with strain LW3. The effects of chlorimuron‐ethyl concentration and temperature on biodegradation were examined. Two metabolites of degradation were analyzed by LC/MS. Based on the identified products, strain LW3 seemed to be able to degrade chlorimuron‐ethyl by cleavage of the sulfonylurea bridge. The inoculation of strain LW3 to chlorimuron‐ethyl‐treated soil resulted in a higher degradation rate than in uninoculated soil, regardless of the soil being sterilized or nonsterilized. This microbial culture has great potential for the bioremediation of soil contaminated with chlorimuron‐ethyl.