Open AccessVaccination with Streptococcus suis serotype 2 recombinant 6PGD protein provides protection against S. suis infection in swine
Author(s) -
Tan Chen,
Liu Manli,
Liu Jinlin,
Yuan Fangyan,
Fu Shulin,
Liu Yuan,
Jin Meilin,
Bei Weicheng,
Chen Huanchun
Publication year - 2009
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2009.01617.x
Subject(s) - streptococcus suis , immunogenicity , adjuvant , vaccination , immunization , biology , serotype , titer , microbiology and biotechnology , virology , recombinant dna , antibody , immunology , virulence , biochemistry , gene
Streptococcus suis serotype 2 ( S. suis 2 or SS2) is the causative agent of several diseases in both pigs and humans. 6‐Phosphogluconate dehydrogenase (6PGD) is a cell surface protein in SS2. In this study, the immunogenicity and protective efficacy of recombinant 6PGD (r6PGD) from SS2 was evaluated in piglets. Immunization with an r6PGD‐containing adjuvant induced a vigorous immunoglobulin G (IgG) response, with the titer of IgG2 being higher than that of IgG1. Immunization of piglets with r6PGD yielded 50% survival upon an intravenous challenge with a lethal dose of SS2. Piglets immunized with the r6PDG vaccine were better protected than those immunized with the adjuvant control. The clinical signs and histopathological changes in the piglets were recorded. Collectively, these results suggest that r6PGD can confer partial protection against SS2 infection and could be useful for the development of subunit vaccines against SS2.