Open AccessApplication of an immunoaffinity‐based preconcentration method for mass spectrometric analysis of the O‐chain polysaccharide of Aeromonas salmonicida from in vitro ‐ and in vivo ‐grown cells
Author(s) -
Wang Zhan,
Liu Xin,
Garduño Elizabeth,
Garduño Rafael A.,
Li Jianjun,
Altman Eleonora
Publication year - 2009
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2009.01612.x
Subject(s) - aeromonas salmonicida , polyclonal antibodies , in vivo , lipopolysaccharide , polysaccharide , in vitro , chemistry , antiserum , microbiology and biotechnology , capillary electrophoresis , chromatography , biochemistry , bacteria , biology , antibody , immunology , genetics
In this study, application of magnetic beads (Dynabeads) coated with Aeromonas salmonicida lipopolysaccharide‐specific polyclonal antisera to MS‐based characterization of bacterial lipopolysaccharides has been evaluated. The results showed that the affinity‐based preconcentration strategy resulted in at least a 100‐fold increase in the detection of sensitivity, affording direct capillary electrophoresis (CE)‐MS analysis of A. salmonicida lipopolysaccharide O‐chain polysaccharide from in vitro‐ cultured cells. Subsequent CE‐MS analysis of in vivo ‐grown cells of A. salmonicida confirmed significant changes in the structure of the lipopolysaccharide O‐chain polysaccharide as a result of in vivo cultivation.