Open AccessA method for the identification of proteins secreted by lactic acid bacteria grown in complex media
Author(s) -
Sánchez Borja,
Chaignepain Sthéphane,
Schmitter JeanMarie,
Urdaci María C.
Publication year - 2009
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2009.01599.x
Subject(s) - lactic acid , bacteria , trichloroacetic acid , gel electrophoresis , polyacrylamide gel electrophoresis , biochemistry , probiotic , chemistry , biology , microbiology and biotechnology , chromatography , enzyme , genetics
Lactic acid bacteria (LAB) are known for their special nutritional requirements, being usually cultured in complex media to achieve optimal growth. In this paper, a protocol based on trichloroacetic acid precipitation of peptides and proteins is presented. The method has been tested on four probiotic LAB strains grown in De Man Rogosa Sharpe (MRS) broth, a complex medium that is often used for the culture of such bacteria. This protocol allowed the detection of 19 proteins after sodium dodecyl sulfate‐polyacrylamide gel electrophoresis, 10 of them being successfully identified by tandem MS. Thereafter, the 10 were found to be secreted or surface associated by bioinformatic means. In conclusion, this work supplies a method for the identification of proteins secreted by LAB, allowing discrimination between the proteins present in the MRS and those produced by probiotic LAB.