Open AccesspfaB products determine the molecular species produced in bacterial polyunsaturated fatty acid biosynthesis
Author(s) -
Orikasa Yoshitake,
Tanaka Mika,
Sugihara Shinji,
Hori Ryuji,
Nishida Takanori,
Ueno Akio,
Morita Naoki,
Yano Yutaka,
Yamamoto Kouhei,
Shibahara Akira,
Hayashi Hidenori,
Yamada Yohko,
Yamada Akiko,
Yu Reiko,
Watanabe Kazuo,
Okuyama Hidetoshi
Publication year - 2009
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2009.01582.x
Subject(s) - docosahexaenoic acid , biosynthesis , eicosapentaenoic acid , escherichia coli , polyunsaturated fatty acid , biochemistry , bacteria , chemistry , biology , enzyme , fatty acid , gene , genetics
When pDHA4, a vector carrying all five pfaA – pfaE genes responsible for docosahexaenoic acid (DHA; 22:6) biosynthesis in Moritella marina MP‐1, was coexpressed in Escherichia coli with the individual pfaA – pfaD genes for eicosapentaenoic acid (EPA; 20:5) biosynthesis from Shewanella pneumatophori SCRC‐2738, both polyunsaturated fatty acids were synthesized only in the recombinant carrying pfaB for EPA synthesis. Escherichia coli coexpressing a deleted construct comprising pfaA, pfaC, pfaD and pfaE for EPA and pfaB for DHA produced EPA and DHA. Both EPA and DHA were detected in bacteria that inherently contained pfa genes for DHA. These results suggest that PfaB is the key enzyme determining the final product in EPA or DHA biosynthesis.