Open AccessFission yeast syt22 protein, a putative Arf guanine nucleotide exchange factor, is necessary for new end take off
Author(s) -
Fujita Atsushi,
Misumi Yoshio
Publication year - 2009
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2009.01566.x
Subject(s) - schizosaccharomyces pombe , guanine nucleotide exchange factor , saccharomyces cerevisiae , schizosaccharomyces , adp ribosylation factor , yeast , microbiology and biotechnology , biology , genetics , cell , gtpase , golgi apparatus
In fission yeast Schizosaccharomyces pombe , the directions of cell growth change from monopolar to bipolar in character, which is known as ‘new end take off’ (NETO). We previously found that arf6p, a member (class III) of the ADP‐ribosylation factor (Arf) family, is necessary for NETO in fission yeast. Here we report the characterization of an S. pombe gene, syt22 + , encoding a putative Arf guanine nucleotide exchange factor (GEF). The syt22 protein contains a Sec7 domain and a PH domain conserved in the mammalian EFA6 GEF family, and has high similarity to Yel1p, which was identified as a GEF for Arf3p (class III Arf) in Saccharomyces cerevisiae. syt22 Δ cells, like arf6 Δ cells, completely failed to undergo NETO. Syt22p uniformly localizes to the cell periphery. Its localization is not dependent on microtubules, actin cytoskeletons or arf6p. We hypothesize that syt22p functions as a GEF for arf6p.