Open AccessMolecular characterization of newly identified IS 3 , IS 4 and IS 30 insertion sequence‐like elements in Mycoplasma bovis and their possible roles in genome plasticity
Author(s) -
Lysnyansky Inna,
Calcutt Michael J.,
BenBarak Idan,
Ron Yael,
Levisohn Sharon,
Methé Barbara A.,
Yogev David
Publication year - 2009
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2009.01562.x
Subject(s) - insertion sequence , genetics , biology , genome , transposable element , recombinase , consensus sequence , transposase , gene , inverted repeat , peptide sequence , recombination
Insertion sequences (ISs) are mobile genetic elements widely distributed among bacteria. Their impact on the bacterial genome is multifold, including transfer of genetic information, shuttle of adaptive traits and influence on the genomic content. As a result, ISs play an important role in the organization, plasticity and evolution of bacterial genomes. In this study, four new IS elements: IS Mbov7 ; IS Mbov4 and IS Mbov5 ; and IS Mbov6 , related, respectively, to the IS 3 , IS 4 and IS 30 gene families, were identified and characterized with respect to inverted repeat (IR) and directly repeated (DR) sequences, putative target specificity and motifs related to transposase function. For instance, IS 30 ‐related IS Mbov6 isoform elements were shown to (1) contain an α‐helix‐turn‐α‐helix homeodomain (HTH), (2) generate long DR and (3) possess target specificity for a palindromic sequence derived from putative rho‐independent transcription terminators. Members of the IS 3 family, which had not been documented previously in Mycoplasma bovis , contain HTH, leucine zipper and AT‐hook motifs, which may be involved in DNA binding. In addition, the availability of the M. bovis PG45 genome sequence allowed us to elucidate the genomic organization of 54 intact or truncated IS elements and their possible effect on the expression of adjacent genes.