Open AccessA culture‐dependent bacterial community structure analysis based on liquid cultivation and its application to a marine environment
Author(s) -
Shigematsu Toru,
Hayashi Mayumi,
Kikuchi Isamu,
Ueno Shigeaki,
Masaki Haruhiko,
Fujii Tomoyuki
Publication year - 2009
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2009.01536.x
Subject(s) - bacteria , marine bacteriophage , biology , 16s ribosomal rna , enumeration , microorganism , phylogenetic tree , microbial population biology , microbiology and biotechnology , community structure , microbiological culture , seawater , ecology , gene , genetics , mathematics , combinatorics
A method for analyzing culture‐dependent bacterial community structure by liquid cultivation was established using 96‐well microplates. Using 96‐well microplates, this method can easily provide accurate enumeration of viable microorganisms and simultaneous separation of bacteria, which allowed us to analyze the bacterial community. Bacteria in diluted surface seawater were separated using 96‐well microplates and cultivated with 1/5 ZoBell 2216E liquid medium. The 98 cultures obtained were subsequently applied to phylogenetic analysis based on 16S rRNA gene sequences. The bacterial diversity, evaluated by the Shannon–Weaver index, was relatively small but comparable to previously reported bacterial communities of several environments. The most abundant group was the family Rhodobacteraceae , which has been frequently detected in marine environments. Most bacteria were phylogenetically related to bacteria or uncultured clones detected in marine environments, but distant from published species. The analysis of bacterial community structure by liquid cultivation would be useful as an alternative culture‐dependent approach.