Open AccessTranscriptome analysis of cadmium response in Ganoderma lucidum
Author(s) -
Chuang HueyWen,
Wang IWen,
Lin ShenYao,
Chang YuehLong
Publication year - 2009
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2009.01533.x
Subject(s) - transcriptome , biology , microbiology and biotechnology , western blot , gene , ganoderma , proteome , complementary dna , gene expression , genetics , ganoderma lucidum , food science
Ganoderma species are white‐rot fungi widespread throughout the world. In this study, a wild isolate of Ganoderma lucidum was first collected and its tolerance was tested in a medium containing 3.0 mM CdCl 2 . The cDNA‐amplified fragment length polymorphism method was conducted to analyze the transcription profiling of this Ganoderma species in response to Cd treatment. In total, 12 925 transcript‐derived fragments (TDFs) were amplified using 256 primer combinations. Forty‐nine differentially expressed TDFs were confirmed by DNA dot‐blot analysis. Northern blot analysis was used to verify the transcription levels of 34 Cd‐inducible TDFs. Sequence analysis indicated that genes involved in reactive oxygen species generation, synthesis of sulfur‐containing metabolites, translation machinery, DNA repair, transporting system, proteolysis pathway, mitochondria function, and cell wall biosynthesis were upregulated by Cd treatment. Our results provide a genome‐wide transcriptome profiling of Cd response in Ganoderma species.