Open AccessIdentification of a mosaic transposable element of Paracoccus marcusii composed of insertion sequence IS Pmar4 (IS As1 family) and an IS 1247 a‐driven transposable module (TMo)
Author(s) -
Szuplewska Magdalena,
Bartosik Dariusz
Publication year - 2009
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2009.01495.x
Subject(s) - transposable element , insertion sequence , transposition (logic) , clone (java method) , biology , sequence (biology) , genetics , gene , genome , linguistics , philosophy
A sacB ‐based trap plasmid was used to clone a mosaic transposable element (TE) in the carotenoid producer Paracoccus marcusii OS22. This element is composed of an insertion sequence (IS) IS Pmar4 (IS As1 family), which contains an inserted functional transposable module (TMo) generated by a copy of IS 1247 a (of the IS 1380 family). Besides IS 1247 a sequences, the TMo also contains the 3′‐end region of a putative α/β hydrolase gene, whose expression might be activated from the P 2 promoter of IS 1247 a. The identification of this novel TE provides evidence that transposition of TMos may change an IS into a more complex element resembling noncomposite transposons.