Open AccessTNF‐α reduces the level of Staphylococcus epidermidis internalization by bovine endothelial cells
Author(s) -
OviedoBoyso Javier,
BravoPatiño Alejandro,
CajeroJuárez Marcos,
ValdezAlarcón Juan J.,
BaizabalAguirre Víctor M.
Publication year - 2009
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2008.01469.x
Subject(s) - internalization , staphylococcus epidermidis , proinflammatory cytokine , tumor necrosis factor alpha , intracellular , microbiology and biotechnology , biology , cytokine , inflammation , immunology , bacteria , staphylococcus aureus , cell , biochemistry , genetics
Staphylococcus epidermidis is an environmental opportunistic pathogen associated with bovine intramammary infections. In bacterial infections, the endothelial tissue plays an important role during inflammation and it is the target of proinflammatory cytokines such as tumor necrosis factor α (TNF‐α). Therefore, this work was designed to explore the effect of TNF‐α on the interaction of S. epidermidis with bovine endothelial cells (BEC). We show that cell signaling activated by TNF‐α caused a marked reduction in the number of intracellular S. epidermidis , suggesting that molecules participating in this pathway were involved in the internalization of this bacterium. We also found that S. epidermidis internalization was not associated with basal levels of nuclear factor kappa B (NF‐κB) activity because the intracellular number of bacteria recovered after treating BEC with the NF‐κB inhibitors, SN50 or BAY 11–7083, was similar to that of the untreated control. Interestingly, inhibition of the basal activity of JNK with SP600125 and p38 with SB203580 caused a decrease in the number of intracellular S. epidermidis . These results suggest that activation of the signaling pathway initiated by TNF‐α could play an important role in the phagocytosis of this bacterium. However, the basal activity of NF‐κB was shown not to be important for the internalization process of S. epidermidis .